What does this Biochemistry cheat sheet cover?

It has 10 sections: Amino Acids, Protein Structure, Enzymes, Inhibition, Carbohydrates, Lipids & Membranes, Glycolysis, Citric Acid Cycle, ETC / OxPhos and Gene Expression. Together they cover the topics a typical Biochemistry exam tests, including amino acids and properties, protein structure levels, enzyme kinetics: Michaelis-Menten and inhibition types.

Is the biochemistry cheat sheet free?

The first part is free to read on this page, with no account needed. The full sheet opens in the app with Unlimited ($4.99 a month or $19 for a semester), where you can edit it, print it or save it as a PDF. It is one US Letter page and prints cleanly in black and white or colour.

Can I bring this Biochemistry cheat sheet to my exam?

Only if your course allows a note sheet, so check the syllabus. This one is a single US Letter page. If your exam allows a different size, such as a 3x5 or 4x6 index card or one page front and back, Cheat Sheet AI makes one at exactly that size from your own lecture notes.

The sheet was written by our engine from a standard course outline, then a second model fact-checked every formula and statement against it; it corrected 4 statements before publishing. Notation and emphasis vary between courses, so check it against your own lecture notes.

Can I make a Biochemistry sheet from my own notes?

Yes. Upload your lecture slides, notes or study guide and Cheat Sheet AI writes a sheet in your course's notation, sized to exactly the space your exam allows. Your first sheets are free.

Chemistry & BioBiochemistry

Biochemistry Cheat Sheet — 1-page PDF

Amino acids, proteins, enzymes, metabolism, membranes, and information flow. The whole course on one dense, printable page for your midterm or final, with every formula typeset and worked examples where they help.

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For a specific exam: Biochemistry Final exam study sheet.

1 pageUS Letter
10 sections10 topics
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Preview of the one-page Biochemistry cheat sheet: Amino acids, proteins, enzymes, metabolism, membranes, and information flow — the whole course, one page

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What’s on it

The topics a typical Biochemistry exam tests, all on the one page.

Amino acids and properties
Protein structure levels
Enzyme kinetics: Michaelis-Menten
Inhibition types
Carbohydrates
Lipids and membranes
Glycolysis
Citric acid cycle
Electron transport and oxidative phosphorylation
DNA replication, transcription, translation

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The free part of the sheet, section by section. Unlock the full sheet to read the rest, edit any block, and print it or save it as a PDF.

01Amino Acids

Amino acid: $α$ -amino carboxylic acid; at physiological pH usually zwitterion $H X_{3} N X^{+} CH (R) COO X^{-}$ .

AA classes

Class	Examples	Key property
Nonpolar	Gly, Ala, Val, Leu, Ile, Met, Phe, Trp, Pro	Hydrophobic
Polar uncharged	Ser, Thr, Asn, Gln, Tyr, Cys	H-bonds
Acidic	Asp, Glu	$-$ charge at pH 7
Basic	Lys, Arg, His	$+$ charge; His near p $K_{a}$

p I = \frac{p K _{a 1} + p K _{a 2}}{2}

For amino acids w/o ionizable side chain; pI = average of p $K_{a}$ values flanking neutral form.

Zwitterion: Molecule with both positive and negative formal charges; net charge may be 0.
Peptide bond: Amide linkage between $α$ -carboxyl and $α$ -amino group; planar, partial double-bond character; usually trans.

Watch out:At pH $=$ pI: net charge $= 0$ but not all groups uncharged; solubility often minimal.

02Enzymes

Efficiency

\frac{k _{c a t}}{K _{m}}

Catalytic efficiency; higher = better at low $[S]$ .

$K_{m}$: Substrate concentration at $v_{0} = \frac{1}{2} V_{ma x}$ ; often inverse proxy for affinity.

Low $[S]$ : $v_{0} \approx (V_{ma x} / K_{m}) [S]$
High $[S]$ : $v_{0} \to V_{ma x}$
Plot: hyperbola; Lineweaver-Burk: $1/ v_{0}$ vs $1/ [S]$

Watch out: $K_{m}$ is not always $K_{d}$ ; only in special kinetic cases.

03Inhibition

Classic inhibition

Type	$V_{ma x}$	$K_{m}$	Can overcome by $[S]$ ?
Competitive	same	up	yes
Pure noncompetitive	down	same	no
Uncompetitive	down	down	no

Competitive: Inhibitor binds active site of free enzyme; substrate and inhibitor compete.
Noncompetitive: Inhibitor binds allosteric site to $E$ and/or $E S$ ; decreases active enzyme.

04Lipids & Membranes

Fatty acid effects

Feature	Effect
More saturation	Higher melting point, tighter packing
More cis unsaturation	Lower melting point, more fluid
Longer chain	Higher melting point

Cholesterol: Membrane buffer of fluidity; decreases permeability; precursor of steroid hormones, bile acids, vitamin D.
Membrane fluidity: Increases with unsaturated tails, shorter tails, higher temperature; cholesterol buffers extremes.

Watch out:Cis double bonds kink tails; trans pack more like saturated fats.

05Glycolysis

Location: Cytosol; glucose $\to$ pyruvate.

Net glycolysis

Glucose + 2 NAD X^{+} + 2 ADP + 2 Pi 2 Pyruvate + 2 NADH + 2 H X^{+} + 2 ATP + 2 H X_{2} O

Net per glucose in aerobic conditions.

06Citric Acid Cycle

Energy

3 NADH + 1 FADH_{2} + 1 GTP

Per acetyl-CoA from one TCA turn.

Watch out:TCA is amphibolic: both catabolic and biosynthetic intermediate source.

07ETC / OxPhos

ETC: Inner mitochondrial membrane electron carriers transfer e $^{-}$ from NADH/FADH2 to O2.
Proton motive force: $Δ p$ from $Δ ψ$ + $Δ$ pH across inner membrane; drives ATP synthase.

Terminal acceptor

\frac{1}{2} O X_{2} + 2 e X^{-} + 2 H X^{+} H X_{2} O

O2 is final electron acceptor in aerobic respiration.

Inhibitors / uncouplers

Agent	Effect
Rotenone	Complex I inhibitor
Antimycin A	Complex III inhibitor
Cyanide / CO	Complex IV inhibitor
Oligomycin	ATP synthase inhibitor
DNP, uncouplers	Collapse gradient; heat

ATP per NADH/FADH2

$\approx 2.5$ ATP per NADH $, \approx 1.5$ ATP per FADH $_{2}$

Modern P/O estimates.

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Protein Structure

Primary
Secondary
Tertiary
Quaternary
Stabilizing forces
Saturation

Enzymes

Michaelis-Menten
Turnover

Inhibition

Uncompetitive

Carbohydrates

Monosaccharide
Anomer
Reducing sugar
Important polysaccharides

Lipids & Membranes

Triacylglycerol
Phospholipid

Glycolysis

Irreversible steps
PFK-1
ATP yield

Citric Acid Cycle

Location
Per acetyl-CoA
Key regulation

ETC / OxPhos

Gene Expression

Replication
Replication proteins
Transcription
Translation
Genetic code

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About the Biochemistry sheet and making your own.

What does this Biochemistry cheat sheet cover?: It has 10 sections: Amino Acids, Protein Structure, Enzymes, Inhibition, Carbohydrates, Lipids & Membranes, Glycolysis, Citric Acid Cycle, ETC / OxPhos and Gene Expression. Together they cover the topics a typical Biochemistry exam tests, including amino acids and properties, protein structure levels, enzyme kinetics: Michaelis-Menten and inhibition types.
Is the biochemistry cheat sheet free?: The first part is free to read on this page, with no account needed. The full sheet opens in the app with Unlimited ($4.99 a month or $19 for a semester), where you can edit it, print it or save it as a PDF. It is one US Letter page and prints cleanly in black and white or colour.
Can I bring this Biochemistry cheat sheet to my exam?: Only if your course allows a note sheet, so check the syllabus. This one is a single US Letter page. If your exam allows a different size, such as a 3x5 or 4x6 index card or one page front and back, Cheat Sheet AI makes one at exactly that size from your own lecture notes.
How accurate is it?: The sheet was written by our engine from a standard course outline, then a second model fact-checked every formula and statement against it; it corrected 4 statements before publishing. Notation and emphasis vary between courses, so check it against your own lecture notes.
Can I make a Biochemistry sheet from my own notes?: Yes. Upload your lecture slides, notes or study guide and Cheat Sheet AI writes a sheet in your course's notation, sized to exactly the space your exam allows. Your first sheets are free.

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