Microbiology Cheat Sheet — 1-page PDF
Bacterial structure, growth, genetics, viruses, fungi/parasitology, antimicrobials, immunity, sterilization. The whole course on one dense, printable page for your midterm or final, with every formula typeset and worked examples where they help.
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For a specific exam: Microbiology Final exam study sheet.
- 1 pageUS Letter
- 10 sections10 topics
- Free previewfull sheet with Unlimited
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What’s on it
The topics a typical Microbiology exam tests, all on the one page.
- Bacterial cell structure
- Gram staining
- Microbial growth curve
- Metabolism
- Bacterial genetics: transformation, transduction, conjugation
- Viruses and replication cycles
- Fungi and parasites
- Antimicrobial mechanisms and resistance
- Innate and adaptive immunity
- Sterilization and disinfection
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The free part of the sheet, section by section. Unlock the full sheet to read the rest, edit any block, and print it or save it as a PDF.
01Cell Structure
- Prokaryote
- Cell w/o nucleus or membrane-bound organelles; DNA in nucleoid; 70S ribosomes.
Major structures
| Structure | Key feature | Function |
|---|---|---|
| Capsule/glycocalyx | External polysaccharide/polypeptide | Adhesion; antiphagocytic |
| Cell wall | Peptidoglycan | Shape; osmotic protection |
| Outer membrane | LPS, porins | Barrier in Gram- |
| Plasma membrane | Phospholipid bilayer | Transport; energy generation |
| Flagella | Flagellin filament | Motility |
| Pili/fimbriae | Protein appendages | Attachment; conjugation |
| Endospore | Dormant tough form | Survival; not reproduction |
Peptidoglycan
N-acetylglucosamine N-acetylmuramic acid rigid mesh
Bacterial cell wall polymer; target of many antibiotics.
- Endospore
- Highly resistant dormant structure formed by some Gram- rods, esp. Bacillus and Clostridium.
- Gram-: thick peptidoglycan; teichoic acids; no outer membrane.
- Gram-: thin peptidoglycan; outer membrane with LPS; periplasm.
- LPS lipid A = endotoxin.
- 70S ribosome = 50S + 30S subunits.
02Growth Curve
- Turbidity
- Indirect measure of cell density in liquid culture; correlates with growth but not viability.
Watch out:Plate count measures viable cells; turbidity counts live + dead particles.
03Metabolism
- Aerobe
- Uses as terminal electron acceptor.
- Anaerobe
- Grows without ; obligate anaerobes may be poisoned by oxygen radicals.
Energy categories
| Mode | Carbon source | Energy source |
|---|---|---|
| Photoautotroph | Light | |
| Chemoautotroph | Oxidation of inorganic compounds | |
| Photoheterotroph | Organic C | Light |
| Chemoheterotroph | Organic C | Organic chemicals |
ATP
Main energy currency; made by substrate-level phosphorylation, oxidative phosphorylation, or photophosphorylation.
- Glycolysis: glucose → pyruvate; ATP + NADH.
- TCA cycle: acetyl-CoA oxidized; NADH/FADH2 produced.
- ETC: e flow drives proton motive force; ATP synthase makes ATP.
04Viruses
Basic parts
| Part | Role |
|---|---|
| Genome | DNA or RNA; ss or ds |
| Capsid | Protein coat; symmetry |
| Envelope | Host-derived lipid membrane; spikes for attachment |
| Spikes | Receptor binding; entry |
- Lytic cycle
- Attachment → entry → biosynthesis → assembly → release; host cell lysed.
- Lysogenic cycle
- Viral genome integrates as prophage/provirus; replicated with host; may later induce lytic cycle.
Baltimore
dsDNA ssDNA dsRNAssRNAssRNA ssRNA-RT dsDNA-RT
Genomes grouped by route to mRNA.
Watch out:Enveloped viruses are generally more fragile to drying, detergents, and heat than nonenveloped viruses.
Ex.Phage integrates into host chromosome, no immediate lysis?
Lysogeny; prophage state.
05Fungi & Parasites
- Fungi
- Eukaryotic heterotrophs with chitin cell walls and ergosterol membranes; yeasts are unicellular, molds filamentous.
06Antimicrobials
- Resistance mechanisms: drug inactivation, target modification, decreased permeability, efflux pumps, bypass pathway, biofilm.
- Beta-lactamases hydrolyze beta-lactam ring.
- MRSA: altered PBP (PBP2a); vancomycin resistance via altered D-Ala-D-Lac target.
Watch out:Misuse selects resistance; biofilms reduce penetration and increase persistence.
07Immunity
- Innate immunity
- Rapid, non-specific, no memory; barriers, phagocytes, complement, inflammation, NK cells.
- Adaptive immunity
- Antigen-specific, clonal expansion, memory; B cells and T cells.
Cells and roles
| Cell | Role |
|---|---|
| Neutrophil | Early phagocyte; acute bacterial infection |
| Macrophage | Phagocytosis; cytokines; antigen presentation |
| Dendritic cell | Major antigen-presenting cell |
| B cell | Antibody production |
| CD4 T | Helper; cytokines |
| CD8 T | Cytotoxic killing |
Antibody
IgM first response→;$ IgA mucosa→$ allergy/parasites
Major immunoglobulin classes.
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Also on the full Microbiology sheet
9 more sections, with every definition, formula and worked example. Unlimited unlocks every full sheet in the library to edit, print or save as a PDF, and makes unlimited sheets from your own notes.
Gram Stain
- Gram stain
- Outcome
Growth Curve
- Binary fission
- Batch culture phases
- Generation time
- Exponential growth
- log growth rate
Metabolism
- Fermentation
Genetics
- Transformation
- Transduction
- Conjugation
- Horizontal gene transfer
- Plasmid
- Operon
Viruses
- Virus
Fungi & Parasites
- Fungal forms
- Parasite
- Key targets
Antimicrobials
- Bactericidal
- Bacteriostatic
- Common mechanisms
- β-lactam
- Selective toxicity
Immunity
- Complement
- Vaccination
Sterilization
- Sterilization
- Disinfection
- Control levels
- Methods
- Pasteurization
FAQ
Questions
About the Microbiology sheet and making your own.
- What does this Microbiology cheat sheet cover?
- It has 10 sections: Cell Structure, Gram Stain, Growth Curve, Metabolism, Genetics, Viruses, Fungi & Parasites, Antimicrobials, Immunity and Sterilization. Together they cover the topics a typical Microbiology exam tests, including bacterial cell structure, Gram staining, microbial growth curve and metabolism.
- Is the microbiology cheat sheet free?
- The first part is free to read on this page, with no account needed. The full sheet opens in the app with Unlimited ($4.99 a month or $19 for a semester), where you can edit it, print it or save it as a PDF. It is one US Letter page and prints cleanly in black and white or colour.
- Can I bring this Microbiology cheat sheet to my exam?
- Only if your course allows a note sheet, so check the syllabus. This one is a single US Letter page. If your exam allows a different size, such as a 3x5 or 4x6 index card or one page front and back, Cheat Sheet AI makes one at exactly that size from your own lecture notes.
- How accurate is it?
- The sheet was written by our engine from a standard course outline, then a second model fact-checked every formula and statement against it; it corrected 4 statements before publishing. Notation and emphasis vary between courses, so check it against your own lecture notes.
- Can I make a Microbiology sheet from my own notes?
- Yes. Upload your lecture slides, notes or study guide and Cheat Sheet AI writes a sheet in your course's notation, sized to exactly the space your exam allows. Your first sheets are free.
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